Chemosensory proteins are associated with thiamethoxam tolerance in bird cherry-oat aphid Rhopalosiphum padi
Ping Gao, Siqian Zhang, Junjie Tan, Xinghao Li and Maohua Chen
Pesticide Biochemistry and Physiology
https://doi.org/10.1016/j.pestbp.2023.105393
Abstract
Rhopalosiphum padi (L.) is an important cosmopolitan pest of cereal crops. Thiamethoxam is widely used for control R. padi in some regions. Chemosensory proteins (CSPs) are a class of transporter proteins in arthropods which play a key role in various physiological processes including response to insecticide exposure. However, the role of R. padi CSPs (RpCSPs) in insecticide binding and susceptibility has not been well clarified. In this study, we found that the expression levels of RpCSP1, RpCSP4, RpCSP5, RpCSP7, RpCSP10 were dramatically upregulated after exposure to thiamethoxam. Suppression of RpCSP4 and RpCSP5 transcription by RNA interference signif-icantly enhanced the susceptibility of R. padi to thiamethoxam. Molecular docking and fluorescence competitive binding showed that RpCSP4 and RpCSP5 had high binding affinity with thiamethoxam. The present results prove that RpCSP4 and RpCSP5 are related to insecticide resistance through high binding affinity to reduce the toxicity of insecticide.