Uridine Diphosphate-Glycosyltransferase RpUGT344D38 Contributes to λ-Cyhalothrin Resistance in Rhopalosiphum padi
Liu, Xi, Suji Wang, Hongcheng Tang, Mengtian Li, Ping Gao, Xiong Peng and Maohua Chen
Journal of Agricultural and Food Chemistry
https://doi.org/10.1021/acs.jafc.3c08403
ABSTRACT
Uridine diphosphate-glycosyltransferase (UGT) is a key phase II enzyme in the insect detoxification system. Pyrethroids are commonly used to control the destructive wheat aphid Rhopalosiphum padi. In this study, we found a highly expressed UGT gene, RpUGT344D38, in both lambda-cyhalothrin (LCR)- and bifenthrin (BTR)-resistant strains of R. padi. After exposure to lambda-cyhalothrin and bifenthrin, the expression levels of RpUGT344D38 were significantly increased in the resistant strains. Knockdown of RpUGT344D38 did not affect the resistance of BTR, but it did significantly increase the susceptibility of LCR aphids to lambda-cyhalothrin. Molecular docking analysis demonstrated that RpUGT344D38 had a stable binding interaction with both bifenthrin and lambda-cyhalothrin. The recombinant RpUGT344D38 was able to metabolize 50% of lambda-cyhalothrin. This study provides a comprehensive analysis of the role of RpUGT344D38 in the resistance of R. padi to bifenthrin and lambda-cyhalothrin, contributing to a better understanding of aphid resistance to pyrethroids.